| Popis: |
The polymerase chain reaction is a powerful tool for deriving semi-quantitative information about the relative abundance of cytokine RNA species that are present in pathologic states, such as autoimmune disease. We describe here the MAPPing technique, by which multiple cytokine RNA species can be analyzed from small tissue samples. A revealing array of information concerning the transcriptional activity of cells can be obtained with this technique. We discuss the qualitative and quantitative aspects of MAPPing that should be considered for optimal performance of the assay and for a constructive interpretation of the results. |
