HPLC Method for Analysis of Red Dye from MarkedPectinophora gossypiella(Lepidoptera: Gelechiidae)

Autor:	Michelle Walters, Nada Tomic-Carruthers, John Claus, Guolei Tang, Noreen Mahoney
Rok vydání:	2013
Předmět:	Chromatography biology Pectinophora gossypiella Isopropyl alcohol Gelechiidae biology.organism_classification High-performance liquid chromatography Lepidoptera genitalia Hexane chemistry.chemical_compound chemistry Insect Science Hplc method Ecology Evolution Behavior and Systematics Pink bollworm
Zdroj:	Florida Entomologist. 96:1574-1578
ISSN:	1938-5102 0015-4040
DOI:	10.1653/024.096.0441
Popis:	We describe an HPLC analytical technique for detection of minute amounts of oil-based Red 26 dye used for marking insects. Chromatographic separation was achieved by an isocratic elution on an Inertsil 100A, 5µ silica column using hexane: isopropyl alcohol (98:2) as the mobile phase and with absorption detection at 520 nm. This newly developed technique is fast and very sensitive, with a detection threshold at 0.1 ng and quantitation down to 0.3 ng per pink bollworm adult. Using the newly developed technique we were able to correlate the titer of dye extracted from adult moths to the amount of dye included in the larval diet. This method is currently accepted by the U.S. cotton industry as a preferred technique for distinguishing artificial diet-reared sterile from wild pink bollworm moths.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9523d0d11654f2458c45544c245550cd https://doi.org/10.1653/024.096.0441 Zobrazit plný text záznamu Plný text ve formátu PDF