Effect of the inhibitors of different steps of cholesterol biosynthesis pathway on high density lipoprotein binding to isolated human enterocytes
| Autor: | D.D. Sviridov, M Y Pavlov, Vadim S. Repin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
biology
Enterocyte Cholesterol Reverse cholesterol transport Pharmaceutical Science Taurocholic acid chemistry.chemical_compound medicine.anatomical_structure Biosynthesis chemistry Biochemistry HMG-CoA reductase medicine biology.protein lipids (amino acids peptides and proteins) Lovastatin medicine.drug Lipoprotein |
| Zdroj: | International Journal of Pharmaceutics. 96:1-4 |
| ISSN: | 0378-5173 |
| Popis: | The effect of cholesterol synthesis inhibitors on high-density lipoprotein (HDL3) binding to isolated human small intestine epithelial cells was studied. Lovastatin (2 nM), ketoconazole (1 μM), miconazole (2 μm), compound U-18666A (1 μgml), 25-hydroxycholesterol (1 μm) and taurocholic acid (2 mM) inhibited [14C]acetate incorporation into cholesterol; lovastatin and ketoconazole also inhibited [14C]oleic acid incorporation into cholesteryl esters. All inhibitors down-regulate HDL3 binding to enterocytes; good correlation was observed between the effects of the compounds on cholesterol synthesis and HDL3 binding. These data allow one to assume that newly synthesized cholesterol rather than cholesterol biosynthesis pathway intermediates is a regulator of HDL receptors. |
| Databáze: | OpenAIRE |
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