Cellular uptake and phototoxicity of surface-modified fluorescent nanodiamonds
| Autor: | Bo-Jui Chang, Huan Niu, Su-Yu Chiang, Niann-Shiah Wang, Mao Feng Weng |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
medicine.diagnostic_test Chemistry Mechanical Engineering Analytical chemistry General Chemistry Receptor-mediated endocytosis Fluorescence Electronic Optical and Magnetic Materials Flow cytometry Cancer cell Materials Chemistry Biophysics medicine MTT assay Electrical and Electronic Engineering Cytotoxicity Phototoxicity |
| Zdroj: | Diamond and Related Materials. 22:96-104 |
| ISSN: | 0925-9635 |
| Popis: | article i nfo Bioconjugated fluorescent nanodiamonds (FNDs) show potential for effectively targeted imaging and the en- hanced photokilling of cancer cells. In this study, we investigated the mechanisms involved in the cellular up- take of surface-modified 140 nm FNDs and evaluated their cytotoxicity and phototoxicity following particle internalization. Through an analysis of flow cytometry, the internalized FND-Tf and FND-NH2 particles reached their respective saturation values, with half-life times of approximately 0.8 h and 1.4 h. The determi- nation indicated that the receptor-mediated endocytosis of FND-Tf particles was highly effective and nearly twice as efficient as the endocytic process of FND-NH2 particles mainly through surface electrostatic interac- tions. The cytotoxicity of internalized particles was evaluated using the MTT assay, indicating that at satura- tion concentrations, the rate of proliferation of FND-treated cells decreases to less than half that of untreated cells. An examination of the phototoxicity of internalized FND-Tf particles under irradiation using a 532 nm laser revealed that the FND-treated cells could be killed using less than half the energy required for untreated cells. The enhanced cellular killing was attributed to FND particles converting light to thermal energy, with the support of Raman spectra of nanodiamond clusters that showed an increase in particle temperature under irradiation. Our results demonstrated that bioconjugated FNDs could be highly effective in the target- ing of cancer cells for fluorescence imaging and photokilling with a minimum of collateral cell damage. © 2011 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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