The use of multivariate analysis for the design of selective isolation conditions for mutants of Streptomyces cattleya with improved antibiotic titre
| Autor: | Adekunle O. Onadipe, Michael E. Bushell |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Genetics
education.field_of_study Streptomyces cattleya biology Renewable Energy Sustainability and the Environment medicine.drug_class Streptomycetaceae General Chemical Engineering Organic Chemistry Population Antibiotics biology.organism_classification medicine.disease_cause Pollution Streptomyces Microbiology Inorganic Chemistry Fuel Technology Discriminant function analysis medicine Actinomycetales education Waste Management and Disposal Bacteria Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 39:237-249 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.280390405 |
| Popis: | Twenty-three tests which did not include antibiotic production were carried out on ultraviolet-induced mutants of Streptomyces cattleya NRRL 8057. Principal component analysis of the results indicated the taxometric relationships between members of a population consisting of 77 isolates. Highlitre antibiotic producers were then marked on the population distribution diagram and clusters containing high-titre antibiotic producers noted. Discriminant analysis for the high-titre clusters provided a discriminant function which was derived from data from seven of the tests. These were used to formulate a positive selection procedure which was successful in selecting for a population of mutants which contained a significantly higher number of high-titre isolates than a control population. |
| Databáze: | OpenAIRE |
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