Genetic diversity ofBacillus thuringiensis serovars revealed by RFLP using random DNA probes

Autor: Kwang-Bo Joung, Stéphan P. Lemaire, Jean-Charles Côté
Rok vydání: 2001
Předmět:
Zdroj: Journal of Basic Microbiology. 41:85-95
ISSN: 1521-4028
0233-111X
DOI: 10.1002/1521-4028(200105)41:2<85::aid-jobm85>3.0.co;2-r
Popis: EcoRI and HindIII restriction fragment length polymorphism (RFLP) profiles using 2 random DNA probes, named 104 and 106, were generated for 85 B. thuringiensis strains. These include 80 serovars, 4 intra-serovar strains: kurstaki HD-1, dendrolimus, tenebrionis and sandiego, and a non-serotypeable strain B. thuringiensis var. wuhanensis. A total of 47 EcoRI and 65 HindIII restriction patterns were generated when hybridization results from both probes were combined. Seventy-seven B. thuringiensis strains showed distinctive hybridization profiles. The dendrogram resulting from the numerical analysis of the distance matrix revealed fourteen distinct phylogenetic groups at the 96% banding patterns similarity. The intra-serovar strains showed higher similarity with their respective type serovars. However, different serovars from a common H-serotype did not always cluster in the same phylogenetic group. Alternatively, several mosquitocidal serovars clustered in a single phylogenetic group. The correlation between serotyping and banding pattern similarity is discussed.
Databáze: OpenAIRE
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