Molecular Characterization of the Vitellogenin Receptor inBactericera cockerelli(Sulc)
| Autor: | Adriana E. Flores, Gustavo Ponce-Garcia, Iram P. Rodriguez-Sanchez, Beatriz Lopez-Monroy, J. Refugio Lomeli-Flores, Humberto Quiroz-Martínez, Susana Favela-Lara, O. Karina Villanueva‐Segura |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Bactericera cockerelli Ecology biology fungi food and beverages Solanum tuberosum biology.organism_classification Molecular biology Zebra chip 03 medical and health sciences Vitellogenin Complete sequence 030104 developmental biology Insect Science GenBank Botany biology.protein PEST analysis Solanum Agronomy and Crop Science |
| Zdroj: | Southwestern Entomologist. 41:605-612 |
| ISSN: | 2162-2647 0147-1724 |
| Popis: | Potato purple top and zebra chip diseases are transmitted by potato psyllid, Bactericera cockerelli (Sulc), to tomato, Solanum lycopersicum L., and potato, Solanum tuberosum L., crops. The aim of this study was to characterize and quantify vitellogenin receptor in potato psyllid. We obtained partial characterization of the receptor by end-point PCR and sequencing using capillary electrophoresis. Relative quantification by real-time PCR was used to determine expression levels of the receptor in females and males. Similarity in vitellogenin sequence obtained in a laboratory was 89% compared with the sequence of the entire transcriptome of the species reported in GenBank. Expression analysis showed the vitellogenin receptor significantly expressed in potato psyllid females compared with males from potato plants. Further study of the vitellogenin receptor of potato psyllid is needed to determine its complete sequence and potential target to control the pest. |
| Databáze: | OpenAIRE |
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