Study of the pre-implantation development of mice embryos marked with a green fluorescent protein (EGFP) gene

Autor:	L. K. Sasina, O. V. Kidgotko, M. M. Shavlovskii, A. P. Dyban, T. D. Aleinikova, S. I. Gorodetskii, E. M. Nonyashvili, Sorokin Av
Rok vydání:	2008
Předmět:	animal structures Pronucleus Transgene embryonic structures Inner cell mass Embryo Cell Biology Biology Enhancer Gene Microinjection Molecular biology Green fluorescent protein
Zdroj:	Cell and Tissue Biology. 2:347-353
ISSN:	1990-5203 1990-519X
Popis:	We present the data on the in vitro development of mouse embryos after injecting pronuclei with cloned DNA fragments with an enhanced green fluorescent protein (EGFP) gene under the control of different regulatory elements (promoters, enhancers, stop signals, etc). It was found that the microinjection procedure itself inhibits development independent of the genetic constructs applied. The development of transgenic embryos may be blocked at different stages of cleavage and morula or blastocyte stages. Most transgenic embryos proved to be mosaics. Transgenic cells are very frequently found in trophectoderm; i.e. they are not a part of the inner cell mass, which is a progenitor for the development of embryo tissues.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::94e65935aa9a3e6011ea6879d2bf3e7c https://doi.org/10.1134/s1990519x08040019 Zobrazit plný text záznamu Full text from SpringerLink