The use of immunothermistography as a method of detecting humoral antibodies following allografting of skin and cornea in rabbits
| Autor: | A. K. Saakov, M. M. Kapichnikov, A. M. Nogaller, L. G. Levkoeva, L. F. Lazarenko |
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| Rok vydání: | 1975 |
| Předmět: |
Pathology
medicine.medical_specialty biology business.industry medicine.medical_treatment General Medicine General Biochemistry Genetics and Molecular Biology Highly sensitive Titer medicine.anatomical_structure Blood serum Cornea Immunology biology.protein Medicine Skin grafting sense organs Antibody business |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 80:948-951 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00789278 |
| Popis: | Humoral antibodies were investigated in 43 rabbits, after allografting of the skin and cornea, by the method of immunothermistography, based on changes in the thermal conductivity of the medium as a result of the antigen-antibody reaction. Within 3–5 days after skin grafting antibodies were detected in the blood serum and their titer rose steadily. Repeated skin grafting at first led to a decrease in the concentration of antibodies, but after the 8th–12th day their titer increased. Antibodies disappeared after 2–3 months in most animals. Similar results were obtained after corneal grafting. A parallel was observed between the thermistographic data and Hoigne's reaction, and also the dynamics of the complement titer. The results showed that immunothermistography is capable of showing the presence of humoral antitissue antibodies at an early stage of the rejection reaction. The method is highly sensitive and technically easy to perform. |
| Databáze: | OpenAIRE |
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