Optimization of the organic solvent-stable asymmetric hydrogen transfer system of Rhodococcus ruber DSM 44541: an activity-growth study
| Autor: | Birgit Kosjek, Bernhard J. Ueberbacher, M.F. Koegl, Tina M. Poessl, Mateja Pogorevc, Ursula Ellmer, S.R. Wallner, Wolfgang Kroutil, Kurt Faber, Sandra F. Mayer, Wolfgang Stampfer, Silvia M. Glueck |
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| Rok vydání: | 2003 |
| Předmět: |
Chromatography
biology Process Chemistry and Technology Bioengineering Bacterial growth equipment and supplies biology.organism_classification complex mixtures Biochemistry Catalysis Cofactor chemistry.chemical_compound chemistry Volume (thermodynamics) biology.protein Acetone Bioreactor Fermentation Rhodococcus Alcohol dehydrogenase |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 22:1-6 |
| ISSN: | 1381-1177 |
| DOI: | 10.1016/s1381-1177(02)00265-5 |
| Popis: | The organic solvent-stable redox-system of Rhodococcus ruber DSM 44541, which allows the efficient oxidation/reduction of sec-alcohols/ketones at the expense of acetone/2-propanol, respectively, as cosubstrate was optimized with respect to a maximum of alcohol dehydrogenase activity during cell growth. Comparison of the fermentation of R. ruber DSM 44541 in shake flasks cultures (1 l flask with 250 ml medium) and in a bioreactor (15 l with 10 l working volume) revealed that the desired organic solvent-stable alcohol dehydrogenase activity reached its maximum during the log phase for the bioreactor. In contrast, in shake flasks the maximum of activity was reached during the stationary phase. |
| Databáze: | OpenAIRE |
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