Analytical Method Development and Validation for biotin from Premixes (solid blend of Multi-Vitamin) by RP-HPLC
| Autor: | Vishwas C. Bhagat, Rajkumar V. Shete, Amit S. Ravetkar, Harshita H. Patel |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vitamin
Materials science Chromatography Correlation coefficient Calibration curve High-performance liquid chromatography Method development Volumetric flow rate chemistry.chemical_compound Biotin chemistry Pharmacology (medical) Acetonitrile Pharmacology Toxicology and Pharmaceutics (miscellaneous) |
| Zdroj: | Research Journal of Pharmacy and Technology. 13:1314 |
| ISSN: | 0974-360X 0974-3618 |
| DOI: | 10.5958/0974-360x.2020.00242.5 |
| Popis: | To Develop and Validate Analytical method which is simple, rapid and sensitive for the determination of biotin from premixes (solid blend of multi-vitamin) by RP-HPLC. RP-HPLC analysis was performed using HPLC Agilent Technology 1260 Infinity series HPLC. The chromatographic separation was obtained by using column C18 Thermo scientific ODS Hypersil, 150×4.6, 3 μ, mobile phase used was buffer: acetonitrile in the ratio 91.5: 8.5, Flow rate 1.2 mi/min, wavelength 200nm, Injection volume 100 micro lit, column oven temp. 40°C, run time: 15 min. The retention time of biotin was found to between 10–12 min. Calibration curve was linear with a correlation coefficient of 0.996 over the concentration range of 50–150 ppm and precise with % RSD < 2 for standard and for sample % RSD < 5. The method was found to be specific, linear, precise and accurate. Suitable for the Quality control analysis. |
| Databáze: | OpenAIRE |
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