Species status evaluation of Lirceus usdagalan, L. culveri, and L. hargeri populations (Isopoda; Asellidae) based on a large scale next-generation sequence data set

Autor:	William Orndorff, David B. Carlini, Daniel W. Fong
Rok vydání:	2021
Předmět:	education.field_of_study biology Lirceus Population Endangered species Zoology biology.organism_classification Intraspecific competition Genetic divergence Species of concern Asellidae Genetics Lirceus usdagalun education Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics. 22:661-667
ISSN:	1572-9737 1566-0621
DOI:	10.1007/s10592-021-01387-6
Popis:	In this study the taxonomic status of three species of freshwater asellid isopod crustaceans, Lirceus hargeri, Lirceus usdagalun, and Lirceus culveri is evaluated using a large-scale RNA-seq data set. Whether each are distinct species, or whether they may be morphological variants of the same species is of particular importance for L. usdagalun, an endangered species, and L. culveri, a species of concern, since synonymy of the two could result in significantly reduced protections for the two taxa. RNA-seq was conducted on pooled individuals from each of two populations of L. usdagalun and L. hargeri, and one population of L. culveri. A single transcriptome was assembled de novo from the reads from all five populations, blasted against the NCBI non-redundant protein database, and sequence analysis was conducted on ≥ 13,468 highly expressed transcripts with significant blastx hits (E
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9498cbbdcfe3226166b58f34f3b0ca3b https://doi.org/10.1007/s10592-021-01387-6 Zobrazit plný text záznamu Full text from SpringerLink