Microbiological Assay of Erythromycin Thiocyanate in Feeds
| Autor: | J A Gordon, J Mannebach, H S Perdue |
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| Rok vydání: | 1969 |
| Předmět: | |
| Zdroj: | Journal of AOAC INTERNATIONAL. 52:672-675 |
| ISSN: | 0004-5756 |
| DOI: | 10.1093/jaoac/52.4.672 |
| Popis: | A modified procedure has been developed for extracting low levels (5-20 g/ton) of erythromycin thiocyanate from feeds. In the new method, erythromycin thiocyanate is extracted from the feed with acetone, the acetone is evaporated, and the extract is washed with n-hexane (5 parts) followed by methanol (1 part). The n-hexane and methanol washings are combined and partitioned with phosphate buffer prior to assay using Sarcina lutea ATCC 9341. Recovery averaged 106 ± 10%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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