Autor: |
Hiroaki Ozaki, Mohammad Mehedi Masud, Tomokazu Masuda, Shingo Kimura |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Letters in Organic Chemistry. 16:122-127 |
ISSN: |
1570-1786 |
DOI: |
10.2174/1570178615666180910111359 |
Popis: |
The incorporation of the modified nucleoside into the 3´-overhang regions of short interfering RNAs (siRNAs) has been reported to enhance their nuclease resistance and improve RNA interference activity. In this study, DNA 2-mers containing C5-polyamine-modified pyrimidine nucleosides were synthesized and then ligated to the 3´-end of the RNA by T4 RNA ligase. The modification of the base moiety with tris(2-aminoethyl)amine affected the ligation efficiency, but the DNA 2-mer containing only one modified nucleoside was ligated with sufficient efficiency. We propose a novel synthetic route to modified siRNA bearing various modified groups in the 3´-overhang region. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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