Sex Hormones Impact Early Maturation and Immune Response in the Arteriovenous Fistula Mouse Model

Autor:	Keyuree Satam, Yuichi Ohashi, Carly Thaxton, Luis Gonzalez, Ocean Setia, Hualong Bai, Yukihiko Aoyagi, Yangzhouyun Xie, Weichang Zhang, Bogdan Yatsula, Kathleen Martin, Yujun Cai, Alan Dardik
Rok vydání:	2023
Předmět:	Physiology Physiology (medical) Cardiology and Cardiovascular Medicine
Zdroj:	American Journal of Physiology-Heart and Circulatory Physiology.
ISSN:	1522-1539 0363-6135
Popis:	Arteriovenous fistulae (AVF) fail to mature in female patients compared with male patients, leading to inferior outcomes and decreased utilization. As our mouse AVF model recapitulates sex differences in human AVF maturation, we hypothesized that sex hormones mediate these differences during AVF maturation. C57Bl/6 mice (9-11 week) underwent aortocaval AVF surgery and gonadectomy. AVF hemodynamics were measured via ultrasound (days 0-21). Blood was collected for FACS, and tissue for immunofluorescence and ELISA (days 3,7); wall thickness was assessed using histology (day 21). Inferior vena cava (IVC) shear stress was higher in male mice (p=0.0028) after gonadectomy, and they had increased wall thickness (22.0 ± 1.8 um vs. 12.7 ± 1.2; p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9468897301a8ca24de81fc8ba137411e https://doi.org/10.1152/ajpheart.00049.2023 Zobrazit plný text záznamu