Ultrastructure and immunolabelling of fungi cells treated with Vigna unguiculata vicilins (7S storage proteins)

Autor:	Kátia Valevski Sales Fernandes, José Xavier-Filho, Flavio Costa Miguens, Maura da Cunha, Valdirene Moreira Gomes, Tatiana L. Rose
Rok vydání:	1998
Předmět:	Hypha Cell growth food and beverages Plant Science General Medicine Fungi imperfecti Biology biology.organism_classification Microbiology Spore Cell wall Biochemistry Fusarium oxysporum Genetics Ultrastructure Spore germination Agronomy and Crop Science
Zdroj:	Plant Science. 138:81-89
ISSN:	0168-9452
Popis:	Scanning electron microscopy showed that vicilins from cowpea seeds affect the growth and inhibit spore germination of Fusarium oxysporum and leads to abnormal development (sporulation) of Saccharomyces cerevisiae cells. Control treatments induced normal hyphal and cell development. Fluorescence microscopy and immunolabelling analysis showed that anti-vicilin serum strongly labelled the cell surfaces of yeast and filamentous fungi, previously incubated with these seed proteins. These results suggest that the inhibitory effects of cowpea vicilins on fungi growth may be exerted through their ability to bind to the cell surfaces of the micro-organisms, cell wall and/or plasma membrane.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::94588089ed70d346650856f80f76ed3c https://doi.org/10.1016/s0168-9452(98)00148-4 Zobrazit plný text záznamu Full Text from ScienceDirect