Comparative Study of Reversed-Phase High-Performance Liquid Chromatography and Ultraviolet–Visible Spectrophotometry to Determine Doxorubicin in pH-Sensitive Nanoparticles

Autor: Ana Isa Pedroso Marcolino, Joana Rodrigues Fernandes, Laís E. Scheeren, Daniele Rubert Nogueira-Librelotto, Letícia B. Macedo, Clarice Madalena Bueno Rolim, M. Pilar Vinardell
Rok vydání: 2018
Předmět:
Zdroj: Analytical Letters. 51:1445-1463
ISSN: 1532-236X
0003-2719
DOI: 10.1080/00032719.2017.1380034
Popis: Analytical methods by reversed-phase high-performance liquid chromatography (HPLC) and ultraviolet–visible spectrophotometry were developed and validated to determine doxorubicin in pH-sensitive chitosan nanoparticles. Chromatographic separation was performed on a reversed-phase C18 column, with ultraviolet detection at 254 nm and a mobile phase composed by 90% (v/v) acetonitrile in water and water pH 3.0 (33:67, v/v). The spectrophotometry method had the wavelength set at 480 nm and pH 3.0 water was used as a diluent. Calibration curves were linear from 1 to 30 µg/ mL (r > 0.9995) and the specificity was demonstrated by verifying the absence of interferences from nanoparticle components. The values of accuracy and precision were within the acceptable limits, and robustness studies were performed by a two-level full factorial design. The validated methods were further tested to assess doxorubicin content in six different batches of pH-sensitive chitosan nanoparticles. The comparative analyses show...
Databáze: OpenAIRE
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