LIGHT ASSISTED IN-VIVO MICROWAVE SENSING FOR ELECTRICAL CHARACTERIZATION OF PROKARYOTES
| Autor: | Rajveer Sharma, Prem Saran Tirumalai, K. S. Daya |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Biophysical Reviews and Letters. :219-227 |
| ISSN: | 1793-7035 1793-0480 |
| DOI: | 10.1142/s1793048012500105 |
| Popis: | This paper reports an in vivo characterization technique to characterize dielectric properties of living tissues and bio-molecules at microwave frequency using cavity perturbation technique, where a slot ring resonant sensor has been used, that works at 8 GHz and has been designed to enumerate the effective dielectric constant of Spirulina platensis and chlorophyll molecule. Observed value of the dielectric constant of Spirulina platensis was 8 ± 0.04 in the absence of light and 14.575 ± 0.145 in the presence of light. Molecular polarizability of chl a molecule was 5.07 ± 0.05 × 104 Å3. Experimentally calculated local electric field actually experienced by chl a molecule was 14.197 ± 0.003 V/m for applied field of 9.79 V/m across the slot ring, dipole moment of chl a molecule was 2.175 ± 0.005 × 105 Debye and total polarisation produced due to these molecules was 1.545 ± 0.005 C/m 2. Observed relaxation time of chl a molecule was 8.09 ± 0.18 × 10-9 s . The proposed sensing method can be an alternate to spectral characterisation technique, generally used to characterize light sensitive bio-molecules and can also be extended to characterize light sensitive bio-molecules in plant cells. |
| Databáze: | OpenAIRE |
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