Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
| Autor: | Changjie Lin, Yunpeng Meng, Shaohua Wei, Dekang Gao, Jing Xia, Zhiwei Gu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system diseases Chemistry Cell Cell migration Transfection medicine.disease_cause medicine.disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology Downregulation and upregulation Annexin Apoptosis 030220 oncology & carcinogenesis Pancreatic cancer medicine Cancer research Pharmacology (medical) Carcinogenesis |
| Zdroj: | OncoTargets and Therapy. 13:1471-1480 |
| ISSN: | 1178-6930 |
| DOI: | 10.2147/ott.s228857 |
| Popis: | Background Pancreatic cancer (PC) is a highly lethal malignancy worldwide. Our previous study indicated that overexpression of USP34 could promote tumor growth in PC cells. Therefore, this study aimed to further investigate the role of USP34 during the tumorigenesis of PC. Methods The level of USP34 in PANC-1 and MiaPaCa-2 cells transfected with USP34-shRNAs was detected by RT-qPCR. Moreover, transwell migration and Annexin V/PI analysis were conducted to detect cell migration and apoptosis, respectively. Results In this study, downregulation of USP34 markedly inhibited proliferation and migration, and induced apoptosis in PANC-1 cells. Moreover, silencing of USP34 obviously downregulated the levels of PRR11 and p-p38 in PANC-1 cells. An in vivo study in nude mice bearing PANC-1 cell xenografts confirmed these results. Conclusion Downregulation of USP34 could inhibit proliferation and migration in PANC-1 cells via inhibiting PRR11, and inactivating p38 MAPK signaling. Therefore, USP34 might be a potential therapeutic target for the treatment of PC. |
| Databáze: | OpenAIRE |
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