Gene Expression in Tomato for Glucosylation of Indole-3-Propionic Acid, a Selective Antibacterial Agent against a Bacterial Wilt Pathogen Pseudomonas solanacearum
| Autor: | Yoshinori Matsuda, Hideyoshi Toyoda, Seiji Ouchi, Mitsue Dogo, Keiko Sekimoto, Hiroyuki Tanpo |
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| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Bacterial wilt fungi Pseudomonas food and beverages macromolecular substances Plant Science Biology biology.organism_classification Molecular biology law.invention Amino acid carbohydrates (lipids) Enzyme chemistry Biochemistry law Gene expression Agronomy and Crop Science Gene Polymerase chain reaction Biotechnology Antibacterial agent |
| Zdroj: | Plant Biotechnology. 14:111-116 |
| ISSN: | 1347-6114 1342-4580 |
| Popis: | To examine the mechanism for in planta detoxification of phytotoxicity of indole-3-propionic acid, the gene expression for glucosylation of this compound was detected in treated tomato seedlings by Northern and in situ hybridization with the specific probe. The probe was obtained by polymerase chain reaction of tomato chromosomal DNA using the primers designed on the basis of amino acid sequences which were highly conserved in several enzymes catalyzing glucosylation. The positive hybridization was intensively detected in stems of the treated tomato seedlings. The increase of hybridized transcript was well coincident with the accumulation of glucosyl indole-3-propionic acid analyzed by thin layer chromatography. The result suggests that the PCR clone obtained from tomato chromosome encodes partial sequences of a gene for glucosyl conjugation of the compound. |
| Databáze: | OpenAIRE |
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