Tissue–diet discrimination factors and turnover of stable carbon and nitrogen isotopes in white-footed mice (Peromyscus leucopus)
| Autor: | Christopher S. RomanekC.S. Romanek, Daniel A. Soluk, Rachel L. DeMotsR.L. DeMots, Aaron J. GregorA.J. Gregor, Karen F. Gaines, James M. Novak |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Canadian Journal of Zoology. 88:961-967 |
| ISSN: | 1480-3283 0008-4301 |
| Popis: | Stable isotope analysis has become an increasingly valuable tool in investigating animal ecology. Here we docu- ment the turnover rates for carbon in the liver, muscle, and whole blood tissue, as well as the tissue-diet discrimination values for carbon and nitrogen isotopes in the liver, whole blood, muscle, and hair, of the white-footed mouse (Peromyscus leucopus (Rafinesque, 1818)). A 168-day diet-switching experiment was conducted with a laboratory population of white- footed mice. The d 13 C values for all tissues deviated less than 1% from those of the diet except for whole blood, which had a slightly higher tissue-diet discrimination factor of 1.8%. All tissues were enriched in 15 N by approximately 3% rela- tive to the diet except for liver tissue, which was 4.5% higher than the dietary d 15 N value. Turnover rates for tissues of white-footed mice were ranked liver > whole blood > muscle. The half-lives calculated for liver tissue differed signifi- cantly between the two diet switches performed in this experiment. We demonstrate that there is potential for variation in tissue-diet discrimination values and tissue turnover rates between even closely related species. These findings highlight the importance of determining species-specific estimates of these parameters prior to the use of stable isotope analysis in field investigations of animal ecology. Resume´ : L'analyse des isotopes stables devient un outil de plus en plus precieux dans l'etude ecologique des animaux. Nous determinons ici les taux de remplacement du carbone dans les tissus du foie, du muscle et du sang entier, ainsi que les valeurs de discrimination tissu-regime des isotopes de carbone et d'azote dans le foie, le sang entier, le muscle et le poil chez la souris a pieds blancs (Peromyscus leucopus (Rafinesque, 1818)). Nous avons meneune experience de change- ment de regime alimentaire de 168 jours avec une population de laboratoire de souris a pieds blancs. Les valeurs de d 13 C dans tous les tissus different de moins de 1 % de celles du regime, exceptedans le sang entier qui possede un facteur de discrimination tissu-regime legerement plus elevede 1,8 %. Tous les tissus sont enrichis en 15 N d'environ 3 % par rap |
| Databáze: | OpenAIRE |
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