Metalloproteinase 11, potential marker and molecular target in advanced and castration-resistant prostate cancer. Culture study of peritumoral fibroblasts
Autor: | M.L. Perez de Haro, J. García-Rodríguez, Jesus Fernandez-Gomez, A. Quintás-Blanco, Noemi Eiro, F. Vizoso-Piñero, C. González-Ruiz de León |
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Rok vydání: | 2017 |
Předmět: |
medicine.drug_class
business.industry 030232 urology & nephrology General Medicine Hyperplasia Androgen medicine.disease 03 medical and health sciences Prostate cancer chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Castration Stroma chemistry Prostate 030220 oncology & carcinogenesis medicine Cancer research Cancer-Associated Fibroblasts Fibroblast business |
Zdroj: | Actas Urológicas Españolas (English Edition). 41:376-382 |
ISSN: | 2173-5786 |
Popis: | Objective To analyze the expression of metalloprotein 11 (MMP11) in cultured fibroblasts obtained from human prostate tumors with different clinical and pathological characteristics. Material and methods For this study we analyzed samples of transrectal prostate biopsies from tumors with different characteristics, treated with or without androgen deprivation (AD). After optimization of the culture method, fibroblasts were isolated and cultured to perform the study (PCR) of MMP11 mRNA. Results Finally, 37 cases were studied: 5 samples of benign prostatic hyperplasia, 14 cases with localized neoplasms (7 high-risk according to the D’Amico classification), 5 with metastasic tumors (bone metastases), and 13 treated with AD therapy, of which 6 fulfilled the requirements to be defined as resistant to castration. In tumors without AD therapy, MMP11 expression was significantly higher (p = 0.001) in fibroblasts of higher grade tumors. A significant (p = 0.001) correlation was found between PSA and expression of MMP11 in fibroblast s and a significant increase of MMP11 expression in metastatic tumors. In tumors with AD therapy, a significantly greater expression of MMP11 was observed in resistant to castration patients than in those sensitive to castration (p = 0.003). Conclusion In advanced prostate tumors or in stages of increased tumor aggressiveness, the production of MMP11 by fibroblasts is significantly greater than in non-metastatic tumors or in AD sensitive tumors. |
Databáze: | OpenAIRE |
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