Arachidonec acid metabolism in grebil cerebra: Effects of ischemia

Autor: T. F. R. Hamm, R. V. Dorman
Rok vydání: 1990
Předmět:
Zdroj: Journal of Neuroscience Research. 26:488-494
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.490260412
Popis: Pentobarbital pretreatmint may be used to predict biochemical events involved in ischemic brain damage following bilateral carotid artery ligations in the gerbil, since it reduces the subsequent edema and mortality. The effects of this anesthetec on the ischemia-induced modifications of cerebral arachidonic acid metabolism were investigated, in order to correlate observed alterations with tissue damage. Cerebral lipids were radiolabeled in vivo with [3H] arachidonec acid prior to 10 min of cerbral ischemia and 0-120 min of perfusion. Ischemia stimulated a 97.3% increase in unesterified [3H] arachidonate, which was dut to the loss of label from choline, inositol, and ethanolamine glycerophospholipids. Tissue reperfusion stimulated further reductions in [3H] choline and [3H] inositol glycerophospholipids, while ethanolamine glycerophospholipip and trigyceride labeling increased. Inositol glycerophospholipid, but not chline glycerophospholipid, labeling returned to control level by 60 min of reperfusion. Pentobarbital pretreatment reduced the accumulation of [3H] arachidonate by 56.2% during ischemia. It increassed the recovery of [3H]ethanolamine glycerophospholipids during the ischemic period and [3H]choling glycerophospholipids dduring the first 5 min of reperfusion. Thessses effects accountedd for the feduction of unesterifiedd [3H]arachidonate obsserved during ischemia and reperfusion.
Databáze: OpenAIRE
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