Efficacy of molecular DNA methods for confirming species identifications on morphologically variable populations of toxin-producingAnabaena(Nostocales)

Autor:	Ann L. St. Amand, Andrew D. Chapman, Mark T. Aubel, J. M. Eilers, Juli Dyble
Rok vydání:	2007
Předmět:	chemistry.chemical_classification Nostocales biology Anabaena Akinete Microcystin Aquatic Science Aphanizomenon biology.organism_classification DNA sequencing chemistry Genetic marker Botany Water Science and Technology Heterocyst
Zdroj:	Lake and Reservoir Management. 23:193-202
ISSN:	2151-5530 1040-2381
DOI:	10.1080/07438140709353922
Popis:	Algal samples were analyzed from 3 lakes, Crane Prairie Reservoir and Odell Lake in Oregon and an Anonymous North East System, using both standard taxonomic criteria for identification and DNA sequencing techniques. Two toxin-producing Anabaena populations, one with consistent akinete structure and another with variable akinete structure, were investigated. Samples were characterized based on several genetic markers (nifH, cpcBA-IGS, ITS1), toxins (anatoxin-a, saxitoxin, and microcystin) and morphological variation. Taxonomy within the Nostacales is based on vegetative and terminal cell structure, filament type and aggregation, and position and structure of heterocysts and akinetes. Many taxonomists rely heavily on akinete structure for microscopic identification. Identification from material preserved with Lugol's solution is challenging due to the breakup of colonies, cell distortion, and masking of pigment color. Based on morphological variation, the Crane Prairie and Odell populations were id...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::941602465c6a3d6f5d520c7dc6455aa5 https://doi.org/10.1080/07438140709353922 Zobrazit plný text záznamu