| Popis: |
Hypochlorous acid (HOCl), the strong oxidising and chlorinating molecule, is produced by myeloperoxidase (MPO), an abundant enzyme in phagocytes, and has been implicated in the pathogenesis of various inflammatory diseases inducing cytotoxic effects. At low concentrations of HOCl, however, the cell priming was revealed. Since MPO in modest quantity was registered in cerebrum, we hypothesized that astrocytes could be targeted by HOCl. Using C6 glioma cells as astrocytes model we have studied HOCl effects on cell morphology and functions. In cell cultivation, HOCl (50–100 μM) was found to affect cell morphological characteristics, including the outgrowths shortening and destruction, intercellular communication disruption as well as cell monolayer exhaustion. At micromolar concentrations of HOCl, the reduction of cell proliferative and mitotic activity (varying its dynamics in different fission phases), apoptosis induction and the enhancement of menadione-induced superoxide generation has been established. The observed effects are not associated with HOCl cytotoxicity. Thus, HOCl at micromolar concentrations could regulate the functions of astrocytes, inducing apoptosis, cytoskeleton reorganization and enhancing the redox activity of the cells. |
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