High frequency lateral flow affinity assay using superparamagnetic nanoparticles
| Autor: | María Carmen Blanco-López, José Ángel Flores García, J.C. Martínez-García, Myriam Oliveira-Rodríguez, D. Lago-Cachón, Montserrat Rivas |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Diffusion 010401 analytical chemistry Nanoparticle Nanotechnology 02 engineering and technology Copper conductor engineering.material 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Magnetic field chemistry.chemical_compound Membrane chemistry engineering Magnetic nanoparticles 0210 nano-technology Electrical impedance Nitrocellulose |
| Zdroj: | Journal of Magnetism and Magnetic Materials. 423:436-440 |
| ISSN: | 0304-8853 |
| DOI: | 10.1016/j.jmmm.2016.09.106 |
| Popis: | Lateral flow assay is one of the simplest and most extended techniques in medical diagnosis for point-of-care testing. Although it has been traditionally a positive/negative test, some work has been lately done to add quantitative abilities to lateral flow assay. One of the most successful strategies involves magnetic beads and magnetic sensors. Recently, a new technique of superparamagnetic nanoparticle detection has been reported, based on the increase of the impedance induced by the nanoparticles on a RF-current carrying copper conductor. This method requires no external magnetic field, which reduces the system complexity. In this work, nitrocellulose membranes have been installed on the sensor, and impedance measurements have been carried out during the sample diffusion by capillarity along the membrane. The impedance of the sensor changes because of the presence of magnetic nanoparticles. The results prove the potentiality of the method for point-of-care testing of biochemical substances and nanoparticle capillarity flow studies. |
| Databáze: | OpenAIRE |
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