Deuterium NMR study of the origin of hydrogen in fatty acids produced in vivo by chicken
| Autor: | Sébastien Pionnier, Stephan Buddrus, Ben-Li Zhang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Deuterium NMR
Hydrogen Stereochemistry chemistry.chemical_element Pentose phosphate pathway 010402 general chemistry 01 natural sciences Industrial and Manufacturing Engineering 03 medical and health sciences chemistry.chemical_compound Biosynthesis 030304 developmental biology 2. Zero hunger chemistry.chemical_classification 0303 health sciences Fatty acid Fructose General Chemistry 3. Good health 0104 chemical sciences Biochemistry chemistry Deuterium Proton NMR Food Science Biotechnology |
| Zdroj: | European Journal of Lipid Science and Technology. 108:125-133 |
| ISSN: | 1438-9312 1438-7697 |
| DOI: | 10.1002/ejlt.200500294 |
| Popis: | The origin of hydrogen in fatty acids produced in vivo by chicken was studied by using isotope tracing monitored by 2 H NMR. Chickens were fed with deuterium-labeled water, deuterium-labeled glucose/fructose and trideuterated acetate. The site-specific isotope distribution of the fatty acids produced in vivo by the chickens was analyzed after transformation into methyl esters. A connection was observed between the hydrogen atoms of the feeds and water and those of the different molecular positions of the fatty acids produced by chicken. Water hydrogen makes an important contribution to the formation of the lipids. The hydrogen of the enzyme-bound acetyl or malonyl moiety in acyl-CoA may be transferred from the C-1, C-2 or C-6 of glucose or fructose and indirectly from water. The hydrogen introduced by the cofactor NADPH may be abstracted from C-1 and C-3 of glucose, through its regeneration in the pentose phosphate metabolism pathway, and contains hydrogen atoms of water. The exogenous acetate enters into the in vivo fatty acid biosynthesis pathway in an efficient way and can make an important contribution to the biosynthesis of the lipids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text