Towards breeding of rapeseed (Brassica napus) with alien cytoplasm and powdery mildew resistance from Ethiopian mustard (Brassica carinata)
| Autor: | Zhen Huang, Xiao-Li Wang, Cheng-Yu Yu, Xiao-Han Zhang, Chun-Yan Dai, Qiong Gong, Ai-Xia Xu, Jun-Gang Dong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Rapeseed biology fungi Brassica carinata Brassica food and beverages Erysiphe cruciferarum Plant Science biology.organism_classification 01 natural sciences Embryo rescue 03 medical and health sciences Horticulture 030104 developmental biology Backcrossing Genetics Agronomy and Crop Science Powdery mildew 010606 plant biology & botany |
| Zdroj: | Breeding Science. 70:387-395 |
| ISSN: | 1347-3735 1344-7610 |
| DOI: | 10.1270/jsbbs.20017 |
| Popis: | Powdery mildew (PM), caused by Erysiphe cruciferarum, is an epidemic of oil rapeseed (Brassica napus L.) growing worldwide, but PM resistant germplasm is rare in this species. We screened 102 accessions of B. napus and other cruciferous species and found an Ethiopian mustard (Brassica carinata) cultivar 'White flower' immune to PM in both the field and greenhouse. Outcrossing in the female parent 'White flower' was promoted by using a chemical gametocide tribenuron-methyl, to obtain hybrid seeds of distant hybridization with an elite B. napus cultivar 'Zhongshuang11'. Three true F1 hybrids with B. carinata cytoplasm were obtained without using embryo rescue, which showed complete male sterility and light yellow petals. The hybrid plants and the progenies derived from backcrossing were validated using morphological traits, seed quality, and molecular markers. Five lines in the BC1F3 generation, named 'W7-1', 'W7-4', 'W7-6', 'W8-1', and 'W8-3', and one BC2F2 line 'W3PS-1', whose young leaf was yellow green, were identified to be resistant or moderately resistant to PM. The seed quality and some morphological traits of these lines resembled the parent 'Zhongshuang11', indicating that the resistance gene(s) has been preliminarily introduced into B. napus. |
| Databáze: | OpenAIRE |
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