Rapid screening, identification, and purification of neuraminidase inhibitors fromLithospermum erythrorhizonSieb.et Zucc. by ultrafiltration with HPLC-ESI-TOF-MS combined with semipreparative HPLC
| Autor: | Yan Huijiao, Li Cui, Ruimin Lv, Hengqiang Zhao, Xiao Wang, Yanling Geng, Zhang Minmin, Daicheng Liu, Jin-Peng Yuan, Daijie Wang, Zhao Zhiguo |
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| Rok vydání: | 2016 |
| Předmět: |
Alkannin
Chromatography biology 010405 organic chemistry Electrospray ionization 010401 analytical chemistry Ultrafiltration Filtration and Separation Nuclear magnetic resonance spectroscopy Lithospermum erythrorhizon biology.organism_classification Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry biology.protein Neuraminidase |
| Zdroj: | Journal of Separation Science. 39:2097-2104 |
| ISSN: | 1615-9306 |
| Popis: | We put forward an efficient strategy based on bioassay guidance for the rapid screening, identification, and purification of the neuraminidase inhibitors from traditional Chinese medicines, and apply to the discovery of anti-influenza components from Lithospermiun erythrorhizon Sieb.et Zucc. Ultrafiltration with high-performance liquid chromatography and electrospray ionization time-of-flight mass spectrometry was employed for the rapid screening and preliminarily identification of anti-influenza components from Zicao. Semipreparative high-performance liquid chromatography was used for the rapid separation and purification of the target compounds. NMR spectroscopy, mass spectrometry, and UV spectroscopy were used for further structural identification, and the activity of the compounds was verified by in vitro assay. Five compounds were found to have neuraminidase inhibitory activity by this method. Subsequently, the five compounds were separated by semipreparative high-performance liquid chromatography with the purity over 98% for all of them by high-performance liquid chromatography test. Combined with the NMR spectroscopy, mass spectrometry, and UV spectroscopy data, they were identified as alkannin, acetylalkannin, isobutyrylalkannin, β,β-dimethylacryloylalkannin and isovalerylalkannin. The in vitro assay showed that all five compounds had good neuraminidase inhibitory activities. These results suggested that the method is highly efficient, and it can provide platform and methodology supports for the rapid discovery of anti-influenza active ingredients from complex Chinese herbal medicines. |
| Databáze: | OpenAIRE |
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