Basic guidelines for the selection of polyelectrolytes that can effectively prevent thermal aggregation of enzymes without any substantial loss in their catalytic activity1

Autor:	V. I. Muronets, S. V. Stogov, Vladimir A. Izumrudov
Rok vydání:	2011
Předmět:	Thermal denaturation chemistry.chemical_classification Polymers and Plastics Chemistry Dehydrogenase General Chemistry Polyelectrolyte Catalysis chemistry.chemical_compound Enzyme Sulfonate Thermal Materials Chemistry Biophysics Organic chemistry Macromolecule
Zdroj:	Polymer Science Series C. 53:97-106
ISSN:	1555-614X 1811-2382
DOI:	10.1134/s1811238211030027
Popis:	Factors governing thermal aggregation and thermal denaturation of a model homotetrametric enzyme, glyceraldehyde-3-phosphate dehydrogenase, electrostatically bound to a polyelectrolyte are revealed. Polyanions with sulfate or sulfonate groups in chains are the most efficient suppressors of aggregation. However, their presence promotes thermal denaturation. Short chains of relatively hydrophobic polyanions exert a strong denaturing effect that changes the secondary, tertiary, and quaternary structures of a protein. Basic guidelines for the selection of a polyelectrolyte are outlined. According to the proposed guidelines, hydrophilic high-molecular-mass macromolecules able to form stable soluble polyelectrolyte complexes with the enzyme are the most efficient at preventing thermal aggregation without substantial loss in catalytic activity.
Databáze:	OpenAIRE
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