Intracytoplasmic sperm injection (ICSI) of prepubertal goat oocytes using fresh and frozen-thawed semen
| Autor: | Irene Menéndez-Blanco, M. G. Catalá, Maria-Teresa Paramio, M. Roura, Anna-Rita Piras, Sandra Soto-Heras, Dolors Izquierdo |
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| Rok vydání: | 2019 |
| Předmět: |
Peanut agglutinin
endocrine system Spermatozoon biology urogenital system 040301 veterinary sciences medicine.medical_treatment Embryogenesis 0402 animal and dairy science Semen 04 agricultural and veterinary sciences 040201 dairy & animal science Intracytoplasmic sperm injection 0403 veterinary science Andrology chemistry.chemical_compound medicine.anatomical_structure Food Animals chemistry Capacitation medicine biology.protein Animal Science and Zoology Propidium iodide Fluorescein |
| Zdroj: | Small Ruminant Research. 170:137-142 |
| ISSN: | 0921-4488 |
| DOI: | 10.1016/j.smallrumres.2018.11.026 |
| Popis: | The aim of this study was to test the effect of fresh and frozen-thawed semen on embryo development of prepubertal goat oocytes fertilized by intracytoplasmic sperm injection (ICSI). Oocytes were in vitro matured in BO-IVM medium for 26 h. Fresh and frozen-thawed semen were selected by centrifugation in BoviPure® commercial gradient. Before injection, spermatozoa were assessed for: capacitation through the detection of the tyrosine phosphorylation, viability through propidium iodide stain, and acrosomal status through fluorescein isothiocyanate-conjugated peanut agglutinin stain. Metaphase II oocytes were injected with a spermatozoon selected by hyaluronic-acid binding medium (SpermSlow ™). Results of frozen-thawed spermatozoa showed significantly higher level of capacitation than fresh spermatozoa (64.92 vs 34.67%, respectively). Moreover fresh semen group showed significantly higher (p |
| Databáze: | OpenAIRE |
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