GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis

Autor: Hao Ma, Haihong Zhao, Xingwang Yu, Yingzi Shen, Yanfeng Jia, Xiaolin Liu, Jiaping Wei
Rok vydání: 2019
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 138:583-595
ISSN: 1573-5044
0167-6857
Popis: Plant annexins are proteins with various functions, such as binding calcium ions and negatively charging lipids. Although their importance in plant stress responses has been widely reported, their function in seeds is rarely studied. A putative annexin was found in developing seed of soybean [Glycine max (L.) Merr] in our previous comparative proteomics study. In this study, it was identified as a soybean annexin, GmANN, through sequence analysis. Quantitative real time PCR (qRT-PCR) analysis indicated that the transcription levels of GmANN were increased significantly under high temperature and humidity (HTH) stress. Moreover, GmANN-transgenic Arabidopsis seeds showed greater resistance and higher seed vitality under HTH stress in contrast to the wild type (WT) seeds. GmANN overexpression plants showed increased peroxidase activities, decreased lipid peroxidation levels and decreased reactive oxygen species release levels compared to the WT plants. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that GmANN interacted with soybean glutathione S-transferase (GmGST). Taken together, our results implied that GmANN might take part in response to HTH stress and be involved in seed vigor formation in plant. GmANN, a soybean annexin, plays an important role in response to plant HTH tolerance and in seed vigor.
Databáze: OpenAIRE
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