Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato
| Autor: | Xiaorui Liu, Guoqian Yang, Li Lin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine UVR8 biology Chemistry Dimer fungi food and beverages biology.organism_classification 01 natural sciences Dissociation (chemistry) UV resistance 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Monomer Biophysics biology.protein Solanum Antibody 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071613696 |
| DOI: | 10.1007/978-1-0716-1370-2_9 |
| Popis: | The UV RESISTANCE LOCUS 8 (UVR8) is a photoreceptor mediating photomorphogenic responses to UV-B. UVR8 exists as homodimer in plants and UV-B induces dissociation of dimeric UVR8 into monomers to initiate responses. The monomer/dimer status of UVR8 is reversible and a dynamic photo-equilibrium is established in plants according to the ambient light conditions. Here we describe a method to detect UVR8 homodimer and monomer by immunoblotting method from tomato (Solanum lycopersicum) plants. The feature of this method is that protein samples are not boiled prior to loading on an SDS-PAGE gel, which allows the detection of UVR8 homodimer and monomers simultaneously with a single antibody. |
| Databáze: | OpenAIRE |
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