Measurement of clomipramine, N-desmethyl-clomipramine, imipramine, and dehydroimipramine in biological fluids by selective ion monitoring, and pharmacokinetics of clomipramine

Autor:	J P Dubois, W Küng, B Wirz, W Theobald
Rok vydání:	1976
Předmět:	Clomipramine Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Liter Desmethyl Pharmacology Imipramine Bioavailability Pharmacokinetics medicine Clomipramine Hydrochloride medicine.drug Whole blood
Zdroj:	Clinical Chemistry. 22:892-897
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/22.6.892
Popis:	To quantitatively determine tricyclic antidepressant agents, we used a combined gas chromatograph/mass spectrometer system, and deuterium-labeled internal standards. Recovery exceeds 95% and the coefficient of variation is less than 4% for human whole-blood samples supplemented with 5 to 15 ng of clomipramine hydrochloride or 20 to 60 ng of dehydroimipramine hydrogen fumarate per milliliter. For both amines, the detection limit is 0.3 mug/liter; Six healthy volunteers who received a single oral dose of 50 mg of clomipramine hydrochloride showed peak drug concentrations in the blood 3 to 5 h after administration, ranging between 14.4 and 30.1 mug/liter. Plasma/whole blood concentration ratios varied from 0.70 to 1.20, and the cumulative renal elimination from 0 to 72 h is less than 0.2% of the dose. This method is suitable for in vivo bioavailability studies of unchanged clomipramine, dehydroimipramine, and imipramine after a single oral dose of as little as 25 mg.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::93b6d743629de61d3960ff21bdcaaeac https://doi.org/10.1093/clinchem/22.6.892 Zobrazit plný text záznamu