Effects of starvation for 4–20 days on the neutral lipid and phospholipid content of Biomphalaria glabrata as determined by high performance thin layer chromatography-densitometry
| Autor: | Ellen Armour, Bernard Fried, Joseph Sherma |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
030110 physiology
0301 basic medicine Chromatography biology Chemistry Silica gel 010401 analytical chemistry Clinical Biochemistry Phospholipid Pharmaceutical Science biology.organism_classification 01 natural sciences Biochemistry Thin-layer chromatography 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Acetic acid Reagent Phosphomolybdic acid Biomphalaria glabrata lipids (amino acids peptides and proteins) High performance thin layer chromatography |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 39:322-324 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826076.2016.1165577 |
| Popis: | The effects of 4, 7, 10, 14, and 20 days of starvation on the neutral and phospholipid levels of Biomphalaria glabrata were determined by high performance thin layer chromatography (HPTLC)-densitometry. Snails were maintained in artificial spring water in jars at 25 ± 1°C. The digestive gland–gonad complex was extracted with chloroform–methanol (2:1), and Analtech laned preadsorbent silica gel HPTLC plates were used with petroleum ether–diethyl ether–glacial acetic acid (80:20:1) mobile phase, phosphomolybdic acid detection reagent, and scanning at 610 nm for neutral lipid analysis, and chloroform–methanol–water (65:25:4.5) mobile phase, cupric sulfate–phosphoric acid reagent, and scanning at 370 nm for phospholipids. Significant differences were observed in the triacylglycerol and steryl ester levels in the starved versus the control snails. |
| Databáze: | OpenAIRE |
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