Data analysis of plasmid stability in continuous culture of recombinantSaccharomyces cerevisiae

Autor:	David A. Mitchell, K. Syamsu, A. Impoolsup, P.F. Greenfield
Rok vydání:	1992
Předmět:	Cell division Saccharomyces cerevisiae Experimental data Biology biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Yeast law.invention Microbiology Plasmid law Recombinant DNA Biological system Quantitative analysis (chemistry) Nonlinear regression
Zdroj:	Biotechnology Techniques. 6:393-398
ISSN:	1573-6784 0951-208X
DOI:	10.1007/bf02447476
Popis:	A simple method using non-linear regression is developed to analyse experimental data from plasmid stability studies of recombinant Saccharomyces cerevisiae grown in continuous cultures with non-selective and selective media. This method simultaneously provides quantitative information on the probability of plasmid loss due to segregation during cell division and the specific growth rates of plasmid-containing and plasmid-free cells at particular dilution rates. The method is applied to a set of experimental data. The three-parameter model, together with the estimated parameter values, provides a good fit to the experimental data.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::93a11e7c611e16d3b7bbdd9d58766a57 https://doi.org/10.1007/bf02447476 Zobrazit plný text záznamu Full text from SpringerLink