Medium Optimization and Proteome Analysis of Protease Production by Janthinobacterium sp
| Autor: | Hyun-do Kim, Jong-il Choi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Gel electrophoresis 0303 health sciences Protease Chemistry medicine.medical_treatment Biomedical Engineering Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Enzyme Biochemistry 010608 biotechnology Proteome medicine Extracellular Viability assay Industrial and production engineering Psychrophile 030304 developmental biology Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 25:787-794 |
| ISSN: | 1976-3816 1226-8372 |
| DOI: | 10.1007/s12257-020-0110-x |
| Popis: | This study describes the optimization of a culture medium to improve protease production by Janthinobacterium sp. using response surface methodology (RSM) and comparative proteomic analysis. Using a one-variable-at-a-time approach, dextrose, Triton X-100, peptone, and pH were identified as significant factors affecting protease production. The optimized medium resulted in a 6.25-fold increase in protease activity when compared to that in the original medium. To investigate the metabolic changes in the optimized medium, extracellular and intracellular proteins were analyzed by two-dimensional gel electrophoresis. Some proteins essential to cell viability were highly expressed in the optimized medium and the outer membrane protein A was secreted at a higher level. These results can be useful for the industrial application of psychrophiles that produce cold-adapted enzymes. |
| Databáze: | OpenAIRE |
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