Production and Characterization of Monoclonal Antibodies against the Poultry Coccidiostat Halofuginone

Autor:	Marcel H. Elissalde, Larry H. Stanker, Ross C. Beier, Loyd D. Rowe
Rok vydání:	1994
Předmět:	Coccidiostat Immunogen Halofuginone biology Chemistry Hydrobromide medicine.drug_class General Chemistry Monoclonal antibody Molecular biology medicine biology.protein Antibody General Agricultural and Biological Sciences Quantitative analysis (chemistry) Keyhole limpet hemocyanin medicine.drug
Zdroj:	Journal of Agricultural and Food Chemistry. 42:1132-1137
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf00041a017
Popis:	A succinyl derivative of halofuginone (Hal) was covalently linked to keyhole limpet hemocyanin to provide an immunogen for production of anti-Hal antibodies. This resulted in six hybridoma cell lines producing Hal-specific monoclonal antibodies (Mabs) of moderate to high affinity. All 6 Mabs were highly specific for Hal relative to 12 compounds with similar structural components. Fifty percent inhibition of control values (IC 50 ) for the Mabs ranged from 0.19 to 12.0 ng of Hal hydrobromide/well. One Mab (IC 50 =0.19 ng/well) was used to develop a competitive enzyme-linked immunosorbent assay (cELISA) for Hal in chicken serum. Interference due to serum matrix effects was effectively eliminated by 4-fold dilution of the sample with assay buffer
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::93979a52c07d9f3fcb13e793591f28a4 https://doi.org/10.1021/jf00041a017 Zobrazit plný text záznamu