Differential Lipid Profiles in Experimental Steatohepatitis: Role for Imaging Mass Spectrometry as a Diagnostic Aid

Autor:	Dale S. Cornett, Suzanne M. de la Monte, Emine B. Yalcin, Kavin M Nunez
Rok vydání:	2015
Předmět:	Phosphatidylethanolamine Alcoholic liver disease Ethanol Phosphatidylserine medicine.disease Molecular biology Psychiatry and Mental health Clinical Psychology chemistry.chemical_compound chemistry Biochemistry Nitrosamine Pi medicine Phosphatidylinositol Steatohepatitis
Zdroj:	Journal of Drug and Alcohol Research. 4:1-11
ISSN:	2090-8342 2090-8334
DOI:	10.4303/jdar/235928
Popis:	Background. Ethanol, tobacco-specific nitrosamine ketone (NNK), and ethanol+NNK exposures cause steatohepatitis, suggesting that smoking can be a cofactor in alcoholic liver disease. Study design. We used MALDI-TOF imaging mass spectrometry (IMS) to characterize hepatic lipid profiles in steatohepatitis caused by ethanol, NNK, and ethanol+NNK. Results. Ethanol, NNK, and ethanol+NNK increased levels and altered the profiles of hepatic phospholipids. Ethanol caused striking accumulations of m/z 932.7 phosphatidylinositol (PI). NNK increased hepatic levels of PIs with m/z's of 934.8, 960.8, and 962.7. Relative abundance of PIs with m/z's of 857.9 or 883.7 increased progressively from control to NNK, then ethanol, and finally ethanol+NNK, indicating differential and additive effects of these exposures. In contrast, no differences occurred with respect to phosphatidylethanolamine (m/z 766.9), phosphatidylserine (m/z 810.9) or PIs with m/z of 960.8 or 962.7. Principal component analysis generated distinct exposure-related hepatic lipid profiles. Conclusion. MALDI-IMS could serve as a complementary diagnostic aid for differentiating underlying causes of steatohepatitis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::939627419bcb560c6fbf966e0b671850 https://doi.org/10.4303/jdar/235928 Zobrazit plný text záznamu