Alginate biosynthesis by Azotobacter bacteria
| Autor: | Anton P. Bonartsev, Garina A. Bonartseva, V. V. Voinova, V. L. Myshkina, E. A. Akulina, T. K. Makhina |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Azotobacter biology Strain (chemistry) biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biosynthesis chemistry 010608 biotechnology medicine Yeast extract Composition (visual arts) Mannitol Aeration Bacteria medicine.drug |
| Zdroj: | Applied Biochemistry and Microbiology. 53:52-59 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | The ability of representatives of various species of the bacterial genus Azotobacter (A. chroococcum 7B, A. chroococcum 12B, A. chroococcum 12BS, A. agile 12, A. indicum 8, A. vinelandii 17, and A. vinelandii 5B) to alginate synthesis has been studied. It has been shown that all tested bacterial strains have this ability to different extents. Capsular alginate comprises from 2.6 to 32% of the total amount of synthesized alginate in various bacterial species. Strains that are able to active synthesis of alginate have been selected; the effect of the medium composition on their biosynthesis has been studied. The optimal conditions for alginate synthesis by the A. chroococcum 12BS producer strain include the presence of mannitol (40 g/L), yeast extract (1%), and low concentration of phosphates (KH2PO4—0.008 g/L, K2HPO4—0.032 g/L) in the medium; alginate production under these conditions is 4.5 g/L. The effect of aeration on polymer biosynthesis has been revealed: an increase in aeration causes an increase in alginate synthesis, while its decrease promotes the synthesis of poly-3-hydroxybutirate. It has been shown by IR spectroscopy that alginates obtained under various conditions of cultivation contain different ratios of residues of mannuronic and guluronic acids (M/G from 70/30 to 80/20) in the polymer chain and also differ in the amount of acetyl groups (from 10 to 25%) in the polyme structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|