| Popis: |
Alpha-amylase is one of the imported enzymes. So, to reduce imports, alpha-amylase indigenous microbes are needed. This study aims to determine the stability of the alpha-amylase from indigenous Lactobacillus plantarum B110 and Leuconostoc mesenteroides EN 17-11 at various storage times and temperatures. Alpha-amylase stability was detected at storage times 0, 7, 14, 21, and 28 days, and in storage temperatures of 27 (room temperature), 4 (cold temperature), and -20 (freezing temperature) degrees C. The unit and relative activities of alpha-amylase were measured using the Bernfeld method. Alpha-amylase stability was measured by the modified Rajshree and Singh method. The research data were statistically analyzed using a Completely Randomized Design (CRD) with three replications. The results showed that the stabilities of alpha-amylase from both bacteria in cold and frozen temperatures at storage times were better than that at room temperature. Stabilities of L.plantarum B110 alpha-amylase in cold temperature at the time of storage amounted to 0.4587-0.8053 U/ml with relative activities of 56.96-100percent, while at freezing temperature 0.5725-1.0143 U/ml and 56.44-100 percent (p less than 0.05). The stabilities of Lc.mesenteroides EN 17-11 alpha- amylase at the cold temperature at the time of storage were 3.3397-4.9534 U/ml with relative activities of 67.42-100 percent, while at freezing temperatures amounted to 3.1492-5.0646 U/ml and 62.18-100 percent (p smaller than 0.05). Based on the unit activity and the relative activity of alpha-amylase, it can be concluded that Lc.mesenteroides EN 17-11 a-amylase had higher activity and stability at cold and freezing temperatures in storage 7-28 days than L.plantarum B110. |
