Heterozygous Mutation of Vegfr3 Reduces Renal Lymphatics without Renal Dysfunction
| Autor: | Quinten Patterson, Reto Fiolka, Saumya Vora, Hao Liu, Denise K. Marciano, Kevin M. Dean, Chitkale Hiremath, Andrew R. Jamieson, Michael T. Dellinger, Zhiguo Shang |
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| Rok vydání: | 2021 |
| Předmět: |
Creatinine
Kidney Pathology medicine.medical_specialty business.industry Kidney development Renal function General Medicine medicine.disease Lymphangiogenesis chemistry.chemical_compound Lymphatic system medicine.anatomical_structure chemistry Nephrology medicine Albuminuria medicine.symptom business Kidney disease |
| Zdroj: | Journal of the American Society of Nephrology. 32:3099-3113 |
| ISSN: | 1533-3450 1046-6673 |
| Popis: | Background Lymphatic abnormalities are observed in several types of kidney disease, but the relationship between the renal lymphatic system and renal function is unclear. The discovery of lymphatic-specific proteins, advances in microscopy, and available genetic mouse models provide the tools to help elucidate the role of renal lymphatics in physiology and disease. Methods We utilized a mouse model containing a missense mutation in Vegfr3 (dubbed Chy) that abrogates its kinase ability. Vegfr3Chy/+ mice were examined for developmental abnormalities and kidney-specific outcomes. Control and Vegfr3Chy/+ mice were subjected to cisplatin-mediated injury. We characterized renal lymphatics using tissue-clearing, light-sheet microscopy, and computational analyses. Results In the kidney, VEGFR3 is expressed not only in lymphatic vessels but also, in various blood capillaries. Vegfr3Chy/+ mice had severely reduced renal lymphatics with 100% penetrance, but we found no abnormalities in BP, serum creatinine, BUN, albuminuria, and histology. There was no difference in the degree of renal injury after low-dose cisplatin (5 mg/kg), although Vegfr3Chy/+ mice developed perivascular inflammation. Cisplatin-treated controls had no difference in total cortical lymphatic volume and length but showed increased lymphatic density due to decreased cortical volume. Conclusions We demonstrate that VEGFR3 is required for development of renal lymphatics. Our studies reveal that reduced lymphatic density does not impair renal function at baseline and induces only modest histologic changes after mild injury. We introduce a novel quantification method to evaluate renal lymphatics in 3D and demonstrate that accurate measurement of lymphatic density in CKD requires assessment of changes to cortical volume. |
| Databáze: | OpenAIRE |
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