Systemic decrease in murine collecting vessel lymphatic function during initial stages of inflammation (110.5)
| Autor: | Melissa Aldrich, Mary Hall, Bonnie Angermiller, Holly Robinson, Eva Sevick-Muraca |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 186:110.5-110.5 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.186.supp.110.5 |
| Popis: | The effects of inflammation on the lymphatic system are largely unknown, with conflicting reports of enhanced or decreased activity. We imaged murine lymphatic vessels and function using a custom-designed ICCD camera/low-level laser system and an intradermally injected fluorescent dye, indocyanine green, which is rapidly taken up by the lymphatic vasculature. Mice injected with LPS exhibited a drastic, systemic decrease in collecting vessel lymphatic propulsive flow velocity and frequency at 4-24 hours after the inflammatory insult, gradually increasing to normal values by 7 days post-inflammation. IL-6, TNF-alpha, MCP-1, MCP-5, RANTES, and IL-10 levels were increased in plasma at 4-24 hours. Draining lymph nodes retained ICG at 6-7 days post-inflammation, perhaps indicating restriction of afferent and/or efferent lymphatic vessels. This system for non-invasive imaging of the lymphatic system under inflammatory conditions may allow insights into mechanisms of inflammation and its resolution. |
| Databáze: | OpenAIRE |
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