Differential expression of akirin gene in black tiger shrimp Penaeus monodon in response to immunostimulant administration and infections with Vibrio harveyi and white spot syndrome virus
| Autor: | D.S. Akhila, Kogaluru Shivakumar Santhosh, Indrani Karunasagar, Moger Rajeish, Madhu K. Mani, Mundanda Muthappa Dechamma, Iddya Karunasagar, Peter Bossier, Biswajit Maiti |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
biology Vibrio harveyi 010604 marine biology & hydrobiology fungi White spot syndrome 04 agricultural and veterinary sciences Aquatic Science biology.organism_classification 01 natural sciences Shrimp Penaeus monodon Microbiology Real-time polymerase chain reaction Gene expression 040102 fisheries 0401 agriculture forestry and fisheries Hepatopancreas Agronomy and Crop Science Gene |
| Zdroj: | Journal of the World Aquaculture Society. 51:1054-1065 |
| ISSN: | 1749-7345 0893-8849 |
| DOI: | 10.1111/jwas.12704 |
| Popis: | The akirin gene, which is strictly localized in the nucleus, plays a critical role in regulating antimicrobial peptide transcription, and has parallel functions to NF-kappa B signaling pathway in both vertebrates and invertebrates. In shrimp, the akirin gene is expressed as innate immunity in response to microbial infection. In the present study, expression of akirin gene in Penaeus monodon with respect to Vibrio harveyi and white spot syndrome virus (WSSV) infections and immunostimulant (beta-glucan) administration were investigated by quantitative polymerase chain reaction. The gene was expressed in various tissue samples of healthy shrimp. Maximum level of expression was immediately after V. harveyi infection, suggesting that it may be an early response gene. Gene expression was remarkably upregulated in the lymphoid organ, gill, and hepatopancreas, whereas downregulation was observed in hemocytes compared with the control. In the case of WSSV-infected samples, the akirin gene was significantly downregulated in the lymphoid organ but there was no significant difference in expression pattern in hemocytes compared to the control. In gill tissue, maximum expression was observed after 2 hr of infection, the same in hepatopancreas. Experimental challenge of beta-glucan fed shrimp infected with V. harveyi and WSSV resulted in significant upregulation of akirin gene expression in lymphoid and gill tissue. |
| Databáze: | OpenAIRE |
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