Observations on the measurement of 2,3- and 2,5-dihydroxybenzoic acid using high performance liquid chromatography

Autor:	B. Aghabeigi, B. Henderson
Rok vydání:	1992
Předmět:	Pharmacology chemistry.chemical_compound Chromatography chemistry Pyrogallol Immunology Pharmacology toxicology Extraction (chemistry) Ethyl acetate Pharmacology (medical) Hydroxyl radical Diethyl ether High-performance liquid chromatography
Zdroj:	Inflammopharmacology. 1:363-368
ISSN:	1568-5608 0925-4692
DOI:	10.1007/bf02755886
Popis:	This paper describes some of the methodological problems associated with the measurement of the aromatic compounds 2,3- and 2,5-dihydroxybenzoic acid (DHB), using high performance liquid chromatography (HPLC). Diethyl ether cannot be recommended for the extraction procedure because the pyrogallol (1,2,3-trihydrobenzene) used as a stabilizer, co-chromatographs with that of 2,5-DHB. Ethyl acetate can be employed for the extraction of 2,3- and 2,5-DHB but only the former can be satisfactorily assayed by HPLC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9340dc57fea76de8a1f5428d65a765a6 https://doi.org/10.1007/bf02755886 Zobrazit plný text záznamu Full text from SpringerLink