Evolution of wax-on-plastic microfluidics for sub-microliter flow dynamics and its application in distance-based assay
Autor: | Mohtashim H. Shamsi, Ahmad Zaman Qamar, Gabriel Parker, Gary R. Kinsel |
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Rok vydání: | 2019 |
Předmět: |
Work (thermodynamics)
Wax Materials science Capillary action 010401 analytical chemistry Flow (psychology) Microfluidics 02 engineering and technology Mechanics 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Viscosity Volume (thermodynamics) Flow velocity visual_art Materials Chemistry visual_art.visual_art_medium 0210 nano-technology |
Zdroj: | Microfluidics and Nanofluidics. 23 |
ISSN: | 1613-4990 1613-4982 |
DOI: | 10.1007/s10404-019-2249-3 |
Popis: | Plastic substrates are known for their flexibility, optical clearance, toughness, heat resistance, and nonabsorbent properties. Here, we introduce new wax-on-plastic microfluidic platforms, which are responsive to liquid types and concentrations in sub-microliter volume regime based on their flow behavior. Fabrication of the wax-on-plastic microfluidic platforms do not require heating to create stable hydrophobic barriers and liquids can move with a capillary flow down to 0.25 μL volume in the microchannels having 10 μm height. The flow dynamics of the studied fluids in these channels followed the Darcy’s theoretical model, which can be correlated with their flow velocity and viscosity. The flow velocity of the liquid flow was used to estimate glucose in simulated urine within ~ 40 s run-time. The distance-based assay involving relying on flow velocities was used to establish a dynamic range of the glucose in simulated urine with limit of quantitation of 0.018%, which is almost 3× lower than the lower limit of glucose quantity normally present in diabetic patients, i.e., 0.05–0.1%. Moreover, these microchannels can also work distinctly with various biofluids, such as sweat, urine, and fat-free milk. |
Databáze: | OpenAIRE |
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