Isolation of Yeasts from Wild Flowers in Gyonggi-do Province and Jeju Island in Korea and the Production of Anti-gout Xanthine Oxidase Inhibitor

Autor:	Jong-Soo Lee, Ha-Kun Kim, Hye Yeon Mun, Hyang Burm Lee, Se Hee Hyun
Rok vydání:	2013
Předmět:	medicine.drug_class Biology medicine.disease Isolation (microbiology) Applied Microbiology and Biotechnology Microbiology Yeast Gout YEPD Genus Pseudozyma chemistry.chemical_compound chemistry Botany medicine Internal transcribed spacer Xanthine oxidase Xanthine oxidase inhibitor Biotechnology
Zdroj:	Korean Journal of Microbiology and Biotechnology. 41:383-390
ISSN:	2234-7305 1598-642X
DOI:	10.4014/kjmb.1309.09002
Popis:	Several yeasts were isolated from flowers found in Gyonggi-do Province and Jeju island in Korea. They were then identified by a comparison of their PCR-amplified D1/D2 regions of 26S rDNA, internal transcribed spacer 1 and 2 inclusive of 5.8S rDNA, using the BLAST database. A total of fifty four yeast strains were isolated from wild flowers in Gyonggi-do and the genus Pseudozyma was noted as being dominant. A total of thirty two strains were isolated from Songaksan and Seongsan-ilchulbong in Jeju island and Sporobolomyces ruberrimus was seen to be dominant. The anti-gout xanthine oxidase inhibitory activities of the culture broths and cell-free extracts from eighty six yeast strains were then determined. The cell-free extracts of Pseudozyma hubeiensis 228-S-1 exhibited the highest xanthine oxidase inhibitory activity of 19.6%. The XOD inhibitor was also maximally produced when Pseudozyma hubeiensis 228-S-1 was cultured at for 36h in YEPD medium.
Databáze:	OpenAIRE
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