Mitotic gene conversion events induced in G1-synchronized yeast cells by gamma rays are similar to spontaneous conversion events

Autor:	Phoebe S. Lee, Thomas D. Petes
Rok vydání:	2010
Předmět:	Loss of heterozygosity Multidisciplinary biology DNA damage Saccharomyces cerevisiae Chromosome Chromatid Gene conversion Ploidy biology.organism_classification Mitosis Molecular biology
Zdroj:	Proceedings of the National Academy of Sciences. 107:7383-7388
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.1001940107
Popis:	In a previous study, we mapped spontaneous mitotic reciprocal crossovers (RCOs) in a 120-kb interval of chromosome V of Saccharomyces cerevisiae . About three-quarters of the crossovers were associated with gene conversion tracts. About 40% of these conversion tracts had the pattern expected as a consequence of repair of a double-stranded DNA break (DSB) of an unreplicated chromosome. We test this hypothesis by examining the crossovers and gene conversion events induced by gamma irradiation in G1- and G2-arrested diploid yeast cells. The gene conversion patterns of G1-irradiated cells (but not G2-irradiated cells) mimic conversion events associated with spontaneous RCOs, confirming our previous conclusion that many spontaneous crossovers are initiated by a DSB on an unreplicated chromosome.
Databáze:	OpenAIRE
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