Dopamine D2 Receptor Antagonist Alters the Testosterone Release and Kisspeptin/GPR54 Signaling in Food-Restricted Rats

Autor: Farrin Babaei-Balderlou, Seyyed Meysam Abtahi-Foroushani, Khatereh Nourmohammadi
Rok vydání: 2021
Předmět:
Zdroj: Iranian Journal of Science and Technology, Transactions A: Science. 45:1879-1886
ISSN: 2364-1819
1028-6276
DOI: 10.1007/s40995-021-01196-z
Popis: It is well established that acute food restriction (FR) has a suppressive effect on the reproductive system that may be mediated through attenuating the kisspeptin/GPR54 signaling in the hypothalamus. Kisspeptin neurons have been shown to express the dopamine D2 receptor (D2R). The aim of the present study was to investigate the effect of chronic food restriction and/or antagonism of D2R by sulpiride on testosterone release and kisspeptin/GPR54 signaling in male rats. Sixty-six male Wistar rats, weighing-200 ± 20 g, were divided into seven groups including control, sham, 25%FR, 50%FR, 75%FR, sulpiride (50 µg/rat i.c.v., once at the end of treatment), 75%FR + sulpiride. FR was applied for 21 days. At the end, the mean serum testosterone level and the hypothalamic mRNAs expression of KiSS1, GPR54, D2R, and dopamine concentration were evaluated. The results demonstrated that the testosterone level significantly decreased in 50% and 75% food-restricted animals, and in sulpiride group, compared to control rats (P 0.05) compared to the control. Sulpiride injection to 75% food-restricted animals significantly decreased kisspeptin mRNA and increased dopamine concentration (P
Databáze: OpenAIRE
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