X-Ray Spectroscopy in Biology and Medicine

Autor:	Paul K. Lund, James C. Mathies
Rok vydání:	1967
Předmět:	X-ray spectroscopy Chemistry Phosphorus Biochemistry (medical) Clinical Biochemistry Radiochemistry Inorganic chemistry chemistry.chemical_element Calcium Sulfur Quantitative determination Ammonium hydroxide chemistry.chemical_compound Calcium phosphorus Quantitative analysis (chemistry)
Zdroj:	Clinical Chemistry. 13:962-969
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/13.11.962
Popis:	An X-ray emission spectrographic procedure for the quantitative analysis of calcium, sulfur, and phosphorus in urinary calculi has been devised. Duplicate quantitative analyses are possible if 150 µg. of specimen is available. The calcium-, phosphorus-, and sulfur-containing compounds in the sample are quantitatively dissolved in 0.05 M disodium ethylenediaminetetraäcetate prepared in N ammonium hydroxide. An aliquot of this solution, representing 50 µg. of sample, is dried as a thin film on an aluminum foil planchet and quantitated for the indicated elements using the X-ray spectrograph. Preparation of uniform dry films is ensured by adding methylcellulose to the solution of calculus salts. The precision of the method permits estimation of the calcium, sulfur, and phosphorus in each specimen to the nearest whole per cent. Analytic data on 245 specimens of urinary calculi from humans are summarized.
Databáze:	OpenAIRE
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